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goat polyclonal anti-oct4 (igg)  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology goat polyclonal anti-oct4 (igg)
    Goat Polyclonal Anti Oct4 (Igg), supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti-oct4+(igg)/oct4+antibody/pm34013645-90-35-40
    Average 90 stars, based on 1 article reviews
    goat polyclonal anti-oct4 (igg) - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: Dynamics of male canine germ cell development.
    Article Snippet: The primary antibodies included polyclonal anti-rabbit IgG anti-DDX4 (VASA) (1:500, ab13840, Abcam, Cambridge, England), polyclonal anti-rabbit IgG anti-DAZL (1:200, ab34139, Abcam, Cambridge, England), polyclonal anti-rabbit IgG anti-DPPA3 (STELLA) (1:500, sc67249, Santa Cruz Biotechnology, CA, USA), polyclonal goat IgG anti-POU51F (OCT4) (1:100, sc-8629, Santa Cruz Biotechnology, CA, USA), polyclonal anti-rabbit IgG anti-SOX2 (1:100, ab97959, Abcam, Cambridge, England), monoclonal mouse IgG anti-5mC (1:500, ab10805, Abcam, Cambridge, England), polyclonal anti-rabbit IgG anti-5hmC (1:100, ap9160a, Abgent, San Diego, CA, USA), polyclonal anti-rabbit IgG anti-H3K27me3 (1:500,7449, Millipore, Temecula, CA, USA), and polyclonal anti-rabbit IgG anti-H3K9me2 (1:500, 7441, Millipore, Temecula, CA, USA).

    Article Title: Molecular signature and colony morphology affect in vitro pluripotency of porcine induced pluripotent stem cells.
    Article Snippet: Overall efficiency of cell reprogramming for porcine fibroblasts into induced pluripotent stem cells (iPSCs) is currently poor, and few cell lines have been established.. This study examined gene expression during early phase of cellular reprogramming in the relationship to the iPSC colony morphology and in vitro pluripotent characteristics.. Fibroblasts were reprogrammed with OCT4, SOX2, KLF4 and c-MYC.

    Article Title: Generation of porcine induced-pluripotent stem cells from Sertoli cells.
    Article Snippet: Accepted Manuscript Generation of porcine induced-pluripotent stem cells from Sertoli cells Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit PII: S0093-691X(18)31094-X DOI: 10.1016/j.theriogenology.2018.12.033 Reference: THE 14825 To appear in: Theriogenology Received Date: 15 May 2018 Accepted Date: 20 December 2018 Please cite this article as: Piyathip Setthawong, Praopilas Phakdeedindan, Narong Tiptanavattana, Sasitorn Rungarunlert, Mongkol Techakumphu, Theerawat Tharasanit, Generation of porcine induced-pluripotent stem cells from Sertoli cells, (2018), doi: 10.1016/j.Theriogenology theriogenology.2018.12.033 This is a PDF file of an unedited manuscript that has been accepted for publication.. As a service to our customers we are providing this early version of the manuscript.. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.

    Electron Microscopy:

    Article Title: Mechanics Regulates Fate Decisions of Human Embryonic Stem Cells
    Article Snippet: A custom-developed MATLAB program (details of the program was described in Ref. ; The MathWorks, Natick, MA) was used to calculate deflection of the PDMS micropost centroid from its unbent, unloaded position, which was then converted to the horizontal traction force by multiplying with the nominal spring constant K of the PDMS micropost. .. Cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature, and then permeabilized with 0.1% Triton X-100 (Roche Applied Science, Indianapolis, IN) for 20 min. To assay Oct4 expression, Oct4 rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) primary antibody, which did not cross-react with Oct4 isoform B, was used and detected by the goat-anti-rabbit Alexa-546 secondary antibody (Invitrogen). .. To examine the expression of E-cadherin, mouse anti-E-Cadherin primary antibody (Invitrogen) was used and detected by the goat-anti-mouse Alexa-647 secondary antibody (Invitrogen).

    Article Title: Mechanics regulates fate decisions of human embryonic stem cells.
    Article Snippet: A custom-developed MATLAB program (details of the program was described in Ref. [23]; The MathWorks, Natick, MA) was used to calculate deflection of the PDMS micropost centroid from its unbent, unloaded position, which was then converted to the horizontal traction force by multiplying with the nominal spring constant K of the PDMS micropost. .. Immunofluorescence staining Cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature, and then permeabilized with 0.1% Triton X-100 (Roche Applied Science, Indianapolis, IN) for 20 min. To assay Oct4 expression, Oct4 rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) primary antibody, which did not cross-react with Oct4 isoform B, was used and detected by the goat-anti-rabbit Alexa546 secondary antibody (Invitrogen). .. To examine the expression of E-cadherin, mouse anti-E-Cadherin primary antibody (Invitrogen) was used and detected by the goat-anti-mouse Alexa-647 secondary antibody (Invitrogen).

    Expressing:

    Article Title: Mechanics Regulates Fate Decisions of Human Embryonic Stem Cells
    Article Snippet: A custom-developed MATLAB program (details of the program was described in Ref. ; The MathWorks, Natick, MA) was used to calculate deflection of the PDMS micropost centroid from its unbent, unloaded position, which was then converted to the horizontal traction force by multiplying with the nominal spring constant K of the PDMS micropost. .. Cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature, and then permeabilized with 0.1% Triton X-100 (Roche Applied Science, Indianapolis, IN) for 20 min. To assay Oct4 expression, Oct4 rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) primary antibody, which did not cross-react with Oct4 isoform B, was used and detected by the goat-anti-rabbit Alexa-546 secondary antibody (Invitrogen). .. To examine the expression of E-cadherin, mouse anti-E-Cadherin primary antibody (Invitrogen) was used and detected by the goat-anti-mouse Alexa-647 secondary antibody (Invitrogen).

    Article Title: Mechanics regulates fate decisions of human embryonic stem cells.
    Article Snippet: A custom-developed MATLAB program (details of the program was described in Ref. [23]; The MathWorks, Natick, MA) was used to calculate deflection of the PDMS micropost centroid from its unbent, unloaded position, which was then converted to the horizontal traction force by multiplying with the nominal spring constant K of the PDMS micropost. .. Immunofluorescence staining Cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature, and then permeabilized with 0.1% Triton X-100 (Roche Applied Science, Indianapolis, IN) for 20 min. To assay Oct4 expression, Oct4 rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) primary antibody, which did not cross-react with Oct4 isoform B, was used and detected by the goat-anti-rabbit Alexa546 secondary antibody (Invitrogen). .. To examine the expression of E-cadherin, mouse anti-E-Cadherin primary antibody (Invitrogen) was used and detected by the goat-anti-mouse Alexa-647 secondary antibody (Invitrogen).

    Blocking Assay:

    Article Title: Reduced oxygen concentration enhances conversion of embryonic stem cells to epiblast stem cells.
    Article Snippet: Recently, an additional type of pluripotent stem cell-line derived from mouse embryos has been established and termed epiblast stem cell (EpiSC), and is expected to be an important tool for studying the mechanisms of maintenance of pluripotency since they depend on basic fibroblast growth factor-MAPK and Activin ASmad2/3 signaling to maintain pluripotency, unlike mouse embryonic stem cells (ESCs).. Further, because of the similarities between mouse EpiSCs and human ESCs, EpiSCs are expected to be effective experimental models for human stem cell therapy.. Recently, study for conversion from ESC state to EpiSC state or reversion from EpiSC state to ESC state has attracted interest since these techniques may lead to increasing the potential of pluripotent stem cells and our knowledge about their developmental status.

    Incubation:

    Article Title: Induced overexpression of OCT4A in human embryonic stem cells increases cloning efficiency.
    Article Snippet: Steven C. Tsai, David F. Chang, Chang-Mu Hong, Ping Xia, Dinithi Senadheera, Lisa Trump, Suparna Mishra, and Carolyn Lutzko Division of Research Immunology and Bone Marrow Transplantation, Department of Pediatrics, The Saban Research Institute of Children’s Hospital Los Angeles, University of Southern California, Los Angeles, California; Division of Experimental Hematology and Cancer Biology, Department of Pediatrics, Children’s Hospital Research Foundation, Cincinnati Children’s Hospital Medical Center, Cincinnati, Ohio; and Division of Regenerative Medicine and Cellular Therapies, Hoxworth Blood Center, University of Cincinnati, Cincinnati, Ohio

    Immunofluorescence:

    Article Title: Mechanics regulates fate decisions of human embryonic stem cells.
    Article Snippet: A custom-developed MATLAB program (details of the program was described in Ref. [23]; The MathWorks, Natick, MA) was used to calculate deflection of the PDMS micropost centroid from its unbent, unloaded position, which was then converted to the horizontal traction force by multiplying with the nominal spring constant K of the PDMS micropost. .. Immunofluorescence staining Cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature, and then permeabilized with 0.1% Triton X-100 (Roche Applied Science, Indianapolis, IN) for 20 min. To assay Oct4 expression, Oct4 rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) primary antibody, which did not cross-react with Oct4 isoform B, was used and detected by the goat-anti-rabbit Alexa546 secondary antibody (Invitrogen). .. To examine the expression of E-cadherin, mouse anti-E-Cadherin primary antibody (Invitrogen) was used and detected by the goat-anti-mouse Alexa-647 secondary antibody (Invitrogen).

    Staining:

    Article Title: Mechanics regulates fate decisions of human embryonic stem cells.
    Article Snippet: A custom-developed MATLAB program (details of the program was described in Ref. [23]; The MathWorks, Natick, MA) was used to calculate deflection of the PDMS micropost centroid from its unbent, unloaded position, which was then converted to the horizontal traction force by multiplying with the nominal spring constant K of the PDMS micropost. .. Immunofluorescence staining Cells were fixed with 4% paraformaldehyde (Electron Microscopy Sciences, Hatfield, PA) for 20 min at room temperature, and then permeabilized with 0.1% Triton X-100 (Roche Applied Science, Indianapolis, IN) for 20 min. To assay Oct4 expression, Oct4 rabbit polyclonal IgG (Santa Cruz Biotechnology, Santa Cruz, CA) primary antibody, which did not cross-react with Oct4 isoform B, was used and detected by the goat-anti-rabbit Alexa546 secondary antibody (Invitrogen). .. To examine the expression of E-cadherin, mouse anti-E-Cadherin primary antibody (Invitrogen) was used and detected by the goat-anti-mouse Alexa-647 secondary antibody (Invitrogen).



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    FIG. 6. Delayed morula-to-blastocyst transition of mutant embryos. A) Left: microscopic images of 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCre þ females. Eight-week-old Mylk1 mutant and control female siblings were mated with wild-type males. Plugged females were sacrificed at 3.5 dpc. Embryos were collected by uterine flushing and then cultured in vitro to 4 dpc. Right: blastocyst rates were scored based on 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCreþ females, respectively. B) Left: confocal microscopic images of blastocysts from Mylk1fl/fl and Mylk1fl/fl;GCreþ females. The collected blastocysts were stained for <t>Oct4</t> (green). DNA was counterstained with DAPI (blue), and morphology was determined by differential interference contrast (DIC) microscopy., Right: the average number of total cell in blastocysts from Mylk1fl/fl and Mylk1fl/
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    FIG. 6. Delayed morula-to-blastocyst transition of mutant embryos. A) Left: microscopic images of 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCre þ females. Eight-week-old Mylk1 mutant and control female siblings were mated with wild-type males. Plugged females were sacrificed at 3.5 dpc. Embryos were collected by uterine flushing and then cultured in vitro to 4 dpc. Right: blastocyst rates were scored based on 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCreþ females, respectively. B) Left: confocal microscopic images of blastocysts from Mylk1fl/fl and Mylk1fl/fl;GCreþ females. The collected blastocysts were stained for <t>Oct4</t> (green). DNA was counterstained with DAPI (blue), and morphology was determined by differential interference contrast (DIC) microscopy., Right: the average number of total cell in blastocysts from Mylk1fl/fl and Mylk1fl/
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    FIG. 6. Delayed morula-to-blastocyst transition of mutant embryos. A) Left: microscopic images of 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCre þ females. Eight-week-old Mylk1 mutant and control female siblings were mated with wild-type males. Plugged females were sacrificed at 3.5 dpc. Embryos were collected by uterine flushing and then cultured in vitro to 4 dpc. Right: blastocyst rates were scored based on 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCreþ females, respectively. B) Left: confocal microscopic images of blastocysts from Mylk1fl/fl and Mylk1fl/fl;GCreþ females. The collected blastocysts were stained for <t>Oct4</t> (green). DNA was counterstained with DAPI (blue), and morphology was determined by differential interference contrast (DIC) microscopy., Right: the average number of total cell in blastocysts from Mylk1fl/fl and Mylk1fl/
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    FIG. 6. Delayed morula-to-blastocyst transition of mutant embryos. A) Left: microscopic images of 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCre þ females. Eight-week-old Mylk1 mutant and control female siblings were mated with wild-type males. Plugged females were sacrificed at 3.5 dpc. Embryos were collected by uterine flushing and then cultured in vitro to 4 dpc. Right: blastocyst rates were scored based on 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCreþ females, respectively. B) Left: confocal microscopic images of blastocysts from Mylk1fl/fl and Mylk1fl/fl;GCreþ females. The collected blastocysts were stained for <t>Oct4</t> (green). DNA was counterstained with DAPI (blue), and morphology was determined by differential interference contrast (DIC) microscopy., Right: the average number of total cell in blastocysts from Mylk1fl/fl and Mylk1fl/
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    FIG. 6. Delayed morula-to-blastocyst transition of mutant embryos. A) Left: microscopic images of 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCre þ females. Eight-week-old Mylk1 mutant and control female siblings were mated with wild-type males. Plugged females were sacrificed at 3.5 dpc. Embryos were collected by uterine flushing and then cultured in vitro to 4 dpc. Right: blastocyst rates were scored based on 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCreþ females, respectively. B) Left: confocal microscopic images of blastocysts from Mylk1fl/fl and Mylk1fl/fl;GCreþ females. The collected blastocysts were stained for <t>Oct4</t> (green). DNA was counterstained with DAPI (blue), and morphology was determined by differential interference contrast (DIC) microscopy., Right: the average number of total cell in blastocysts from Mylk1fl/fl and Mylk1fl/
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    FIG. 6. Delayed morula-to-blastocyst transition of mutant embryos. A) Left: microscopic images of 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCre þ females. Eight-week-old Mylk1 mutant and control female siblings were mated with wild-type males. Plugged females were sacrificed at 3.5 dpc. Embryos were collected by uterine flushing and then cultured in vitro to 4 dpc. Right: blastocyst rates were scored based on 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCreþ females, respectively. B) Left: confocal microscopic images of blastocysts from Mylk1fl/fl and Mylk1fl/fl;GCreþ females. The collected blastocysts were stained for <t>Oct4</t> (green). DNA was counterstained with DAPI (blue), and morphology was determined by differential interference contrast (DIC) microscopy., Right: the average number of total cell in blastocysts from Mylk1fl/fl and Mylk1fl/
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    Image Search Results


    FIG. 6. Delayed morula-to-blastocyst transition of mutant embryos. A) Left: microscopic images of 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCre þ females. Eight-week-old Mylk1 mutant and control female siblings were mated with wild-type males. Plugged females were sacrificed at 3.5 dpc. Embryos were collected by uterine flushing and then cultured in vitro to 4 dpc. Right: blastocyst rates were scored based on 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCreþ females, respectively. B) Left: confocal microscopic images of blastocysts from Mylk1fl/fl and Mylk1fl/fl;GCreþ females. The collected blastocysts were stained for Oct4 (green). DNA was counterstained with DAPI (blue), and morphology was determined by differential interference contrast (DIC) microscopy., Right: the average number of total cell in blastocysts from Mylk1fl/fl and Mylk1fl/

    Journal: Biology of reproduction

    Article Title: Deletion of Mylk1 in oocytes causes delayed morula-to-blastocyst transition and reduced fertility without affecting folliculogenesis and oocyte maturation in mice.

    doi: 10.1095/biolreprod.114.122127

    Figure Lengend Snippet: FIG. 6. Delayed morula-to-blastocyst transition of mutant embryos. A) Left: microscopic images of 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCre þ females. Eight-week-old Mylk1 mutant and control female siblings were mated with wild-type males. Plugged females were sacrificed at 3.5 dpc. Embryos were collected by uterine flushing and then cultured in vitro to 4 dpc. Right: blastocyst rates were scored based on 3.5 and 4 dpc embryos from Mylk1fl/fl and Mylk1fl/fl;GCreþ females, respectively. B) Left: confocal microscopic images of blastocysts from Mylk1fl/fl and Mylk1fl/fl;GCreþ females. The collected blastocysts were stained for Oct4 (green). DNA was counterstained with DAPI (blue), and morphology was determined by differential interference contrast (DIC) microscopy., Right: the average number of total cell in blastocysts from Mylk1fl/fl and Mylk1fl/

    Article Snippet: Antibodies used in our experiments were available from the following companies: rabbit monoclonal anti-MLCK antibody (Abcam); rabbit polyclonal anti-phospho-myosin light chain 2 (pMLC2) (Ser19) antibody (CST); mouse monoclonal anti-b-actin antibody (Santa Cruz); goat polyclonal anti-Oct4 antibody (Santa Cruz); rabbit polyclonal anti-Bub3 antibody (Santa Cruz); rabbit monoclonal anti-a-tubulin antibody (CST); fluorescein isothiocyanate (FITC)-conjugated rabbit anti-goat immunoglobulin G (IgG) and FITCconjugated goat anti-rabbit IgG (Zhongshan Golden Bridge Biotechnology); and Cy5-conjugated goat anti-rabbit IgG (Jackson ImmunoResearch Laboratory).

    Techniques: Mutagenesis, Control, Cell Culture, In Vitro, Staining, Microscopy